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Concentrações elevadas de NaCl melhoram a criotolerância e a competência do desenvolvimento de oócitos suínos

Vejam, no artigo abaixo (em inglês) a associação entre a concentração do NaCl aumentada - e, por conseqüência, um aumento da pressão osmótica do meio de cultura - como um fator de estresse, temporário, benéfico ao oócito suíno. Tal procedimento, segundo os autores, deveria ser futuramente testado em embriões e gametas de diferentes espécies de mamíferos. A tempo...uma vez mais, seguindo as inovadoras técnicas do WOW, do "submarino", do HMC e da OPS, quem assina o artigo, como autor sênior, é o Gabor Vajta!


Elevated NaCl concentration improves cryotolerance and developmental competence of porcine oocytes

L Lin1,5,4,7, Y Du1,5,4, Y Liu1, PM Kragh1,5, J Li1, S Purup2, M Kuwayama3, X Zhang4, H Yang4, L Bolund5, G Vajta6
1Population Genetics and Embryology, Institute of Genetics and Biotechnology, University of Aarhus, DK-8830 Tjele, Denmark
2Nutrition and Production Physiology, Institute of Animal Health, Welfare and Nutrition, Faculty of Agricultural Sciences, University of Aarhus, DK-8830, Tjele, Denmark
3Advanced Medical Research Institute of Fertility, Kato Ladies Clinic, Shinjuku-ku, Tokyo, 160-0023, Japan
4Beijing Genomics Institute, Airport Industrial Zone B-6, Beijing 101300, China
5Institute of Human Genetics, University of Aarhus, DK-8000, Aarhua, Denmark
6PIVET Medical Centre, 166-168 Cambridge Street, Leederville, Perth, WA 6007, Australia
7Correspondence: Institute of Genetics and Biotechnology, Faculty of Agricultural Sciences, University of Aarhus, Blichers Alle 20, postboks 50, 8830 Tjele, Denmark Tel: 45 8999 1149 Fax: 45 8999 1300 e-mail: mailto:"Lin.Lin@agrsci.dk"

High hydrostatic pressure has been reported to improve the fertilizing or developmental ability of mammalian spermatozoa, oocytes and embryos. This study investigated the effect of another stress, temporarily increased NaCl concentration, on cryotolerance and developmental competence of porcine oocytes. In Experiment 1, survival rates were compared after 1 h exposure to seven elevated NaCl concentrations and 1 h recovery time. In Experiment 2, oocytes were exposed to 593 and 1306 mOsmol NaCl, subsequently recovered, vitrified, then subjected to parthenogenetic activation. Both cleavage and blastocyst rates increased after NaCl treatment compared with untreated controls. In Experiment 3, oocytes were treated with 593 mOsmol NaCl followed by 1 and 2 h recovery, respectively, then used as recipients for somatic cell nuclear transfer (SCNT). Cleavage rates were not different from those in untreated controls, but blastocyst rates increased in both NaCl-treated groups. In conclusion, treatment of porcine oocytes with elevated NaCl concentrations improved their developmental competence after vitrification and parthenogenetic activation or SCNT. Further experiments are required to investigate in-vivo consequences, and the effect on gametes and embryos of different mammalian species.

Reproductive BioMedicine Online 2009 http://www.rbmonline.com/Article/3527 [e-pub ahead of print on 16 January 2009]

Reproductive BioMedicine Online 2009 Vol. 18 No.3. 360–366

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